High-Throughput/High-Content flow cytometery screening service
Typically plates with pre-processed samples (cells treated
according to experimental conditions and stained by the customer
for the desired fluorescent output) are either dropped off or shipped to
our facility. For local customers (San Diego area), we can pickup
samples from and deliver data to your facility. Our scientists will
analyze the samples and generate a single data file in .fcs format
(listmode) and a timing file for each plate. The resulting raw data is either
uploaded to an FTP server for download or burned onto a DVD and
shipped back to the customer. We supply our proprietary analysis
software for effortless final data processing and reporting by the
customer. At your request, we can also assist with building of Excel analysis templates
to enable reporting of the analyzed data in a custom format. We can also
perform data analysis for you, providing raw data files along with analyzed results.
For customers that have developed their own flow cytometer-based assay but would
rather prefer not to deal with compound handling we offer an assay transfer and accommodation
option. Saryna scientists can do cell/tissue culture work, compound handling, staining, and sample analysis in a seamless fashion.
Our laboratory is equipped with Tecan EVO 200 liquid handler which
can be configured for various assays that do not require sterile
environment. It features an 8-channel independently deployable pipetting
head, a 96 channel head, a plate transfer arm, on-board plate
centrifuge and plate lidder/de-lidder. Saryna is also well positioned to provide custom built hardware/electronics to accommodate unusual assay techniques.
- Acceptable volume from 2µL to 900µL.
- Typical volume from 10µL to 50µL.
- Flow rate from 0.1µL/sec to 2.5µL/sec.
- Processing speed up to 70,000 events/sec.
- Supplied in 96 or 384 V bottom plates, tubes for > 250µL volumes.
- Typically < 0.05% with fast washing.
- This number can be further reduced by longer wash time at the expense of throughput.
- Beckman Coulter CyAn ADP flow cytometer.
- 3 lasers - 405nm, 488nm, and 642nm
- Up to 11 readout parameters for each cell/particle.
- Sensitivity: MESF FITC < 100, MESF PE < 50
Using samples with higher cell concentrations reduces the volume required to be injected to achieve necessary number of cells for proper statistics and shortens processing time, thus increasing throughput.
Typical fluorochromes detected by standard filters in our flow cytometer
|Laser Light Source
||Typical Fluorochromes Detected
||FITC, GFP, CFSE, Alexa Fluor 488, YFP
||ECD, PE-Alexa Fluor 610, PI, Qdot605
||PC5, PerCP, PC5.5, PerCP-Cy5.5, 7-AAD, Qdot655
||PC7, PE-Alexa Fluor 750, Qdot705
||Pacific Blue, Cascade Blue, DAPI, CFP
||Cascade Yellow, Pacific Orange, AmCyan, Qdot565
||APC-Cy7, APC-Alexa Fluor 700, APC-Cy5.5, Alexa Fluor